A simple, rapid, and reliable HPTLC method has been established for simultaneous estimation of Nebivolol and Hydrochlorothiazide in combined dosage form. Chromatographic separation was performed on aluminum Precoated with silica gel 60 F254 using Ethyl Acetate: Methanol: Formic acid (8:0.5:0.5 v/v/v) as Mobile Phase. Calibration curves were linear in the range of 600-1400 ng /spot for nebivolol (R2=0.997) and 1500-3500 ng/spot for Hydrochlorothiazide (R2=0.993). Detection was carried out densitometrically at 280 nm. The suitability of this HPTLC method for quantitative determination of the compounds was proved by validation in accordance with the requirements of the ICH guidelines. The method was used for routine analysis of Nebivolol and Hydrochlorothiazide in Tablet dosage form.
Loading....